BRCC36 Antibody

BRCC36 Antibody

Catalog# : 4331

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Additional Names : BRCC36, BRCA1/BRCA2-containing complex subunit 3, BRCC3

Description
Left: Western blot analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemistry of BRCC36 in human brain tissue with BRCC36 antibody at 2.5 µg/ml.

Other Product Images

Source : BRCC36 antibody was raised against a 18 amino acid peptide from near the amino terminus of human BRCC36.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BRCC36 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : BRCC36 antibody can be used for detection of BRCC36 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4331P - BRCC36 Peptide
Long-Term Storage : BRCC36 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1219 - MCF7 Cell Lysate
Species Reactivity :H, M, R
GI Number : 13236583
Accession Number : NP_077308
Short Description : BRCA1/BRCA2-containing complex subunit 3
References
1. Dong Y, Hakimi MA, Chen X, et al. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalsome-like subunit and its role in DNA repair. Mol. Cell 2003; 12:1087-99.
2. Chen X, Arciero CA, Wang C, et al. BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation. Cancer Res. 2006; 66:5039-46.