Bub1 Antibody

Bub1 Antibody

Catalog# : 4239

Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
Additional Names : Bub1 (CT), Budding uninhibited by benzimidazoles 1
Description
Left: Western blot analysis of bub1 in A-20 cell lysate with bub1 antibody at (A) 1 and (B) 2 µg/ml. Below: Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 5 µg/ml.




Source : Bub1 antibody was raised against a 22 amino acid peptide from near the carboxy terminus of human Bub1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bub1 antibody was raised in rabbit.
Application : Bub1 antibody can be used for detection of bub1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4239P - Bub1 Peptide
Long-Term Storage : Bub1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity :H R
GI Number : 8134347
Accession Number : O43683
Short Description : (CT) a kinase involved in spindle formation
References

1. Pangilinan F, Li Q, Weaver T, et al. Mammalian BUB1 protein kinases: map positions and in vivo expression. Genomics 1997; 46:379-88.
2. Williams GL, Roberts TM and Gjoerup OV. Bub1: Escapades in a cellular world. Cell Cycle 2007; 6:1699-704.
3. Meraldi P and Sorger PK. A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J. 2005; 24:1621-33.
4. Tang Z, Shu H, Oncel D, et al. Phosphorylation of cdc20 by bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint. Mol. Cell 2004; 16:387-97.