AP2M1 Antibody

AP2M1 Antibody

Catalog# : 5161

The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
Additional Names : AP2M1, Adaptor-related protein complex 2 mu 1, AP-2 mu-1, mu2, AP50, CLAPM1
Description
Left: Western blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of AP2M1 in mouse kidney tissue with AP2M1 antibody at 2.5 μg/ml.

Other Product Images
Source : AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : AP2M1 antibody was raised in chicken. Please use anti-chicken secondary antibodies.
Application : AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5161P - AP2M1 Peptide
Long-Term Storage : AP2M1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity :H, M, R
GI Number : 14917109
Accession Number : NP_004059
Short Description : Adaptor-related protein complex 2 mu 1
References
1. Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol. 2004; 14:167-74.
2. Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem. 1997; 272:27160-6.
3. Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem. 1994; 269:31592-7.
4. Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology 271:9-17.