Catalog# : 4329
BRCC45 was initially suggested to be a housekeeping protein that is highly expressed in brain and reproductive organs. Later experiments indicated BRCC45 forms a complex with the breast and ovarian predisposition proteins BRCA1 and BRCA2 as well as RAD51 and BRCC36. This complex has a ubiquitin E3 ligase activity and is thought to enhance cellular survival following DNA damage. BRCC45 has also been suggested to function as a death receptor-associated anti-apoptotic protein by inhibiting the BID-induced activation of the mitochondrial apoptotic pathway. Higher levels of BRCC45 were detected in the majority of hepatocellular carcinomas, suggesting that BRCC45 may promote tumorigenesis when overexpressed. At least three isoforms of BRCC45 are known to exist.
Additional Names : BRCC45, BRCA1/BRCA2-containing complex subunit 45, Brain and reproductive organ expressed, BRE, TNFRSF1A modulator
DescriptionLeft: Western blot analysis of BRCC45 in HeLa cell lysate in (A) the absence and (B) presence of blocking peptide with BRCC45 antibody at 1 µg/ml.
Below: Immunohistochemistry of BRCC45 in human brain tissue with BRCC45 antibody at 2.5 µg/ml.
Other Product Images
Source : BRCC45 antibody was raised against a 18 amino acid peptide from near the amino terminus of human BRCC45.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : BRCC45 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : BRCC45 antibody can be used for detection of BRCC45 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4329P - BRCC45 Peptide
Long-Term Storage : BRCC45 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M, R
GI Number : 21361171
Accession Number : NP_004890
Short Description : BRCA1/BRCA2-containing complex subunit 45
References
1. Li L, Yoo H, Beckker FF, et al. Identification of a brain- and reproductive-organs-specific gene responsive to DNA damage and retinoic expression. Biochim. Biophys. Res. Commun. 1995; 206:764-74.
2. Dong Y, Hakimi MA, Chen X, et al. Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalsome-like subunit and its role in DNA repair. Mol. Cell 2003; 12:1087-99.
3. Li Q, Ching AK, Chan BC, et al. A death receptor-associated anti-apoptotic protein, BRE, inhibits mitochondrial apoptotic pathway. J. Biol. Chem. 2004; 279:52106-16.
4. Chan BC, Ching AK, To KF, et al. BRE is an antiapoptotic protein in vivo and overexpressed in human hepatocellular carcinoma. Oncogene 2007; epub.
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