CDC42 Antibody

CDC42 Antibody

Catalog# : 5101

CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to S. cerevisiae CDC42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. CDC42 is thought to regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. At least two isoforms of CDC42 are known to exist.

Additional Names : CDC42, Cell division cycle 42, G25K

Description
Left: Western blot analysis of CDC42 in human brain tissue lysate with CDC42 antibody at (A) 0.5 and (B) 1 µg/ml.




Source : CDC42 antibody was raised against a 17 amino acid peptide near the amino terminus of human CDC42.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CDC42 antibody was raised in chicken. Please use anti-chicken secondary antibodies.
Application : CDC42 antibody can be used for detection of CDC42 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5101P - CDC42 Peptide
Long-Term Storage : CDC42 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity :H, M, R
GI Number : 4757952
Accession Number : NP_001782
Short Description : Cell division cycle 42
References
1. Erickson JW and Cerione RA. Multiple roles for Cdc42 in cell regulation. Curr. Opin. Cell Biol. 2001; 13:153-7.
2. Chen W, Lim HH, and Lim L. The CDC42 homologue from Caenorhabditis elegans. Complementation of yeast mutation. J. Biol. Chem. 1993; 268:13280-5.
3. Hart MJ, Eva A, Evans T, et al. Catalysis of guanine nucleotide exchange on the CDC42Hs protein by the dbl oncogene product. Nature 1991; 354:311-4.
4. Rohatgi R, Ma L, Miki H, et al. The interaction between N-WASP and the Arp2/3 complex links Cdc42-dependent signals to actin assembly. Cell 1999; 97:221-31.