MED28 Antibody

MED28 Antibody

Catalog# : 4731

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin’s tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
Additional Names : MED28, Mediator of RNA polymerase II transcription subunit 28, magicin, EG1
Description
Left: Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below: Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 μg/ml.

Other Product Images
Source : MED28 antibody was raised against a 17 amino acid peptide from near the center of human MED28.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MED28 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : MED28 antibody can be used for detection of MED28 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4731P - MED28 Peptide
Long-Term Storage : MED28 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity :H, M
GI Number : 52851391
Accession Number : NP_079481
Short Description : Mediator of RNA polymerase II transcription subunit 28
References
1. Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell 2004; 14:685-91.
2. Beyer KS, Beauchamp RL, Lee MF, et al. Mediator subunit MED28 (Magicin) is a repressor of smooth muscle cell differentiation. J. Biol. Chem. 2007; 282:32152-7.
3. Wiederhold T, Lee MF, James M, et al. Magicin, a novel cytoskeletal protein associates with the NF2 tumor suppressor merlin and Grb2. Oncogene 2004; 23:8815-25.
4. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.