TRIM5 gamma Antibody

TRIM5 gamma Antibody

Catalog# : 3239

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5gamma variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. Furthermore, the TRIM5gamma isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).
Additional Names : TRIM5 gamma, TRIM5g
Description
Left: Western blot analysis of TRIM5 gamma expression in human bladder (A) and colon (B) cell lysate with TRIM5 gamma antibody at 2 µg /ml.

Below: Immunohistochemistry of TRIM5 gamma in human bladder tissue with TRIM5 gamma antibody at 2 µg/ml.

Other Product Images
Source : TRIM5 gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy-terminus of human TRIM5 gamma.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TRIM5 gamma antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human TRIM5 Gamma Peptide (Cat. No. 3239P)
Application : TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 1 to 2 µg/ml.A band at approximately 35 kDa can be detected.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3239P - TRIM5 gamma Peptide
Long-Term Storage : TRIM5 gamma antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1310 - Human Bladder Tissue Lysate
Species Reactivity :H
GI Number : 15011944
Accession Number : NP_149083
Short Description : Retroviral restriction factor
References
1. Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
2. Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5 restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3. Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5alpha. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9
4. Xu L, Yang L, Moitra PK, et al. BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5. Exp. Cell Res. 2003; 288:84-93.