CCDC98 Antibody

CCDC98 Antibody

Catalog# : 4321

CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.
Additional Names : CCDC98 (NT), Coiled-coil domain 98, Abraxas 1, ABRA1

Description
Left: Western blot analysis of CCDC98 in human breast tissue lysate in (A) the absence and (B) presence of blocking peptide with CCDC98 antibody at 1 µg/ml.

Below: Immunohistochemistry of CCDC98 in human breast tissue with CCDC98 antibody at 5 µg/ml.

Other Product Images

Source : CCDC98 antibody was raised against a 15 amino acid peptide from near the amino terminus of human CCDC98.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CCDC98 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4321P - CCDC98 Peptide
Long-Term Storage : CCDC98 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1311 - Human Breast Tissue Lysate
Species Reactivity :H, M
GI Number : 109148531
Accession Number : NP_620775
Short Description : (NT) coiled-coil domain 98
References
1. Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science 2007; 316:1194-1198.
2. Kim H, Huang J, and Chen J. CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response. Nat. Struct. Mol. Biol. 2007; 14:710-5.
3. Liu Z, Wu J, and Yu X. CCDC98 targets BRCA1 to DNA damage sites. Nat. Struct. Mol. Biol. 2007; 14:716-20.