TRIM5 delta Antibody

TRIM5 delta Antibody

Catalog# : 3241

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region (1). TRIM5 has five alternately spliced isoforms, the longest of which is the ? variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1). Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 (2,3), albeit at differing efficiencies. The TRIM5delta isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5delta also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner (4).
Additional Names : TRIM5 delta, TRIM5d
Description
Left: Western blot analysis of TRIM5 delta expression in mouse brain cell lysate with TRIM5 delta antibody at 2 µg/ml.

Below: Immunohistochemistry of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 1 µg/ml.

Other Product Images

Source : TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy-terminus of human TRIM5 delta.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TRIM5 delta antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human TRIM5 delta Peptide (Cat. No. 3241P)
Application : TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 1 to 2 µg/ml.A band at approximately 25 kDa can be detected.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3241P - TRIM5d Peptide
Long-Term Storage : TRIM5 delta antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity :H
GI Number : 15011946
Accession Number : NP_149084
Short Description : retroviral restriction factor
References
1. Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.
2. Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5 restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3. Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5alpha. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9
4. Xu L, Yang L, Moitra PK, et al. BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5. Exp. Cell Res. 2003; 288:84-93.