IRF8 Antibody

IRF8 Antibody

Catalog# : 3401

Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms (1 for review). During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases (2,3), but IRF8 mediates activation of NF-kappaB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells (4,5). Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia (6).
Additional Names : IRF8

Description
Left: Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1µg/ml in (A) the presence and (B) absence of blocking peptide.






Source : IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy-terminus of human IRF8.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : IRF8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human IRF8 Peptide (Cat. No. 3401P)
Application : IRF8 antibody can be used for detection of IRF8 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3401P - IRF8 Peptide
Long-Term Storage : IRF8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1314 - Human Thymus Tissue Lysate
Species Reactivity :H, M, R
GI Number : 4504567
Accession Number : NP_002154
Short Description : interferon regulatory factor 8
References

1. Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
2. Weisz A, Marx P, Sharf R, et al. The human interferon consensus sequence binding protein (H-ICSBP) is a negative regulator of enhancer elements common to interferon inducible genes. J. Biol. Chem. 1992; 267:25589-96.
3. Nelson N, Marks MS, Driggers PH, et al. Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. Mol. Cell. Biol. 1993; 13:588-99.
4. Tsujimura H, Tamura T, Kong HJ, et al. Toll-like receptor 9 signaling activates NF-kappaB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells. J. Immunol. 2004; 172:6820-7.