Catalog# : 4661
Bst2 was first identified as a novel terminal B-cell-restricted antigen termed HM1.24 that is expressed on certain bone marrow cells as well as other tissues. The surface expression of Bst2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line, suggesting that this protein is involved in pre-B-cell growth. Later experiments also showed it to be upregulated in several myeloma cells. More recently, Bst2 was identified an inhibitor of retroviral release from human cells whose activity is antagonized by the HIV-1 accessory protein Vpu. While Bst2 caused retention of virions on cell surfaces or endocytosis into Bst2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release. This activity may represent a potential therapeutic strategy for the treatment of HIV/AIDS.
Additional Names : Bst2 (NT), Bone marrow stromal cell antigen 2, CD317, HM1.24, tetherin
DescriptionLeft: Western blot analysis of Bst2 in Daudi cell lysate with Bst2 antibody at (A) 1 and (B) 2 µg/ml.
Source : Bst2 antibody was raised against a 18 amino acid peptide near the amino terminus of the mouse Bst2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bst2 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Bst2 antibody can be used for detection of Bst2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4661P - Bst2 Peptide
Long-Term Storage : Bst2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity :H, M
GI Number : 56971124
Accession Number : AAH87949
Short Description : (NT) Bone marrow stromal cell antigen 2
References
1. Goto T, Kennel SJ, Abe M, et al. A novel membrane antigen selectively expressed on terminally differentiated human B cells. Blood 1994; 84:1922-30.
2. Ishikawa J, Kaisho T, Tomizawa H, et al. Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth. Genomics 1995; 26:527-34.
3. Ohtomo T, Sugamata Y, Ozaki Y, et al. Molecular cloning and characterization of a surface antigen preferentially overexpressed on multiple myeloma cells. Biochem. Biophys. Res. Commun. 1999; 258:583-91.
4. Neil SJ, Zang T, and Bieniasz PD. Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu. Nature 2008; 451:425-30.
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