CPEB1 Antibody

CPEB1 Antibody

Catalog# : 4687

CPEB1 is an RNA binding protein that contains an RNA-recognition motif and a zinc finger-containing region found in a wide range of vertebrates and invertebrates. CPEB1 forms the nucleus of a complex of factors that regulate poly(A) elongation and promotes polyadenylation-induced translation. CPEB1 mediates many diverse biological processes such as germ cell development, cell division and senescence, and synaptic plasticity. Recently, it was discovered that CPEB1 is involved in beta-catenin mRNA translation and cell migration in astrocytes as well as regulating hypoxia-inducible factor (HIF)-1 expression, demonstrating the wide range of processes in which CPEB1 plays a role. At least four isoforms of CPEB1 are known to exist.
Additional Names : CPEB1 (NT), Cytoplasmic polyadenylation element-binding protein 1, CPE-B1, CEBP

Description
Left: Western blot analysis of CPEB1 in rat brain tissue lysate with CPEB1 antibody at (A) 1 and (B) 2 µg/ml.




Source : CPEB1 antibody was raised against a 14 amino acid peptide from near the amino terminus of human CPEB1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CPEB1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CPEB1 antibody can be used for detection of CPEB1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4687P - CPEB1 Peptide
Long-Term Storage : CPEB1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity :H, M, R
GI Number : 74762720
Accession Number : Q9BZB8
Short Description : (NT) Cytoplasmic polyadenylation element-binding protein 1
References
1. Richter JD. CPEB: a life in translation. Trends in Biochem. Sci. 2007; 32:179-85.
2. Jones KJ, Korb E, Kundel MA, et al. CPEB1 regulates beta-catenin mRNA translation and cell migration in astrocytes. Glia 2008; 56:1401-13.
3. Hagele S, Kuhn U, Boning M, et al. Cytoplasmic polyadenylation element binding protein (CPEB)1 and 2 bind to the HIF-1alpha mRNA 3’UTR and modulate HIF-1 alpha protein expression. Biochem. J. 2008; epub.