CIP75 Antibody

CIP75 Antibody

Catalog# : 5267

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.
Additional Names : CIP75, Connexin-43-interatcting protein 75, ubiquilin-4, UBQLN4, Ataxin-1 ubiquitin-like-interacting protein, A1U, UBIN
Description
Left: Western blot analysis of CIP75 in 3T3 cell lysate with CIP75 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of CIP75 in mouse kidney tissue with CIP75 antibody at 5 μg/ml.

Other Product Images

Source : CIP75 antibody was raised against an 18 amino acid peptide near the amino terminus of human CIP75.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : Polyclonal
Host : CIP75 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CIP75 antibody can be used for detection of CIP75 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5267P - CIP75 Peptide
Long-Term Storage : CIP75 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity :H, M, R
GI Number : 45476982
Accession Number : Q9NRR5
Short Description : Connexin-43-interatcting protein 75
References
1. Su V and Lau AF. Ubiquitin-like and ubiquitin-associated domain proteins: significance in proteasomal degradation. Cell. Mol. Life Sci. 2009; 66:2819-33.
2. Davidson JD, Riley B, Burright EN, et al. Identification and characterization of an ataxin-1-interacting protein: A1Up, a ubiquitin-like protein. Hum. Mol. Genet. 2000; 9:2305-12.
3. Li X, Su V, Kurata WE, et al. A novel Connexin43-interacting protein, CIP75, which belongs to the UbL-UBA protein family, regulates the turnover of Connexin4 J. Biol. Chem. 2008; 283:5748-59.