CXCR4-Lo Antibody

CXCR4-Lo Antibody

Catalog# : 4443

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV binding sites. This antibody is specific for the longer isoform of CXCR4.
Additional Names : CXCR4-Lo, Fusin, LESTR, HUMSTR
Description
Left: Western blot analysis of CXCR4 in (A) human spleen and (B) human thymus tissue lysate with CXCR4-Lo antibody at 10 µg/ml.

Below: Immunohistochemistry of CXCR4Lo in HeLa cells with CXCR4Lo antibody at 2 µg/ml.

Other Product Images



Source : CXCR4-Lo antibody was raised against a peptide corresponding to nine amino acids near the amino terminus of human CXCR4 isoform a.
Purification : Purified IgG
Clonality and Clone : This is a polyclonal antibody.
Host : CXCR4-Lo antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CXCR4-Lo antibody can be used for Western blot at 5 – 1 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4443P - CXCR4-Lo Peptide
Long-Term Storage : CXCR4-Lo antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 56790927
Accession Number : NP_001008540
Short Description : Isoform a of HIV & chemokine receptor
References
1. Dimitrov DS. Cell 1997; 91:721-30.
2. Feng Y et al. Science 1996; 272:872-7.
3. Berson JF et al. J. Virol. 1996; 70:6288-95.
4. Doranz BJ et al. Cell 1996; 85:1149-58.