CX3CL1 Antibody

CX3CL1 Antibody

Catalog# : 2099

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-alpha in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1. CX3CL1 is ofen observed migrating at 80 – 100kDa in SDS-PAGE, presumably due to post-translational modification.
Additional Names : CX3CL1, CX3C, CX3C membrane-anchored chemokine, neurotactin, small
Description
Left: Western blot analysis of CX3CL1 in C2C12 cell lysate with CX3CL1 antibody at (A) 0.5, (B) 1 and (C) 2 mg/ml.


Source : CX3CL1 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human CX3CL1 .
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CX3CL1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CX3CL1 antibody can be used for detection of CX3CL1 by Western blot at 0.5 – 1 mg/ml dilution. (
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2099P - CX3CL1 Peptide
Long-Term Storage : CX3CL1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1285 - C2C12 Cell Lysate
Species Reactivity :H, M
GI Number : 1899259
Accession Number : AAB50014
Short Description : CX3CR1 ligand
References
1. Bajetto A, Bonavia R, Barbero S, et al. Chemokines and their receptors in the central nervous system. Front. Neuroendocrinol. 2001; 22:147-84.
2. Umehara H, Goda S, Imai T, et al. Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1. Immunol. Cell. Biol. 2001; 79:298-302.
3. Mizuno T, Kawanokuchi J, Numata K, et al. Production and neuroprotective functions of fractalkine in the central nervous system. Brain Res. 2003; 979:65-70.
4. Combadiere C, Salzwedel K, Smith ED, et al. Identification of CX3CR1. A chemotactic receptor for the human CX3C chemokine fractalkine and a fusion coreceptor for HIV-1. J. Biol. Chem. 1998; 273:23799-804.