Bub3 Antibody

Bub3 Antibody

Catalog# : 4227

The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
Additional Names : Bub3 (CT), Budding uninhibited by benzimidazoles 3, Bub3L
Description
Left: Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 µg/ml.

Other Product Images

Source : Bub3 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human bub3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bub3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4227P - Bub3 Peptide
Long-Term Storage : Bub3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity :H, M
GI Number : 7387554
Accession Number : O43684
Short Description : (NT) a kinase involved in spindle formation
References
1. Kalitsis P, Earle E, Fowler KJ, et al. Bub3 gene disruption in mice reveals essential mitotic spindle checkpoint function during early embryogenesis. Genes Dev. 2000; 14:2277-82.
2. Taylor SS, Ha E and McKeon F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol. 1998; 142:1-11.
3. Warren CD, Brady DM, Johnston RC, et al. Distinct chromosome segregation roles for spindle checkpoint proteins. Mol. Biol. Cell 2002; 13: 3029-41.
4. Roberts BT, Farr KA and Hoyt MA. The Saccharomyces cerevisiae checkpoint gene BUB1 encodes a novel protein kinase. Mol. Cell. Biol. 1994; 14:8282-91.