GFR alpha 1 Antibody

GFR alpha 1 Antibody

Catalog# : 1133

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRa, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRalpha-1 recently. GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.
Additional Names : GFR alpha 1 (IN), GFR alpha 1, GFRa-1, GDNFRa, GDNFRalpha
Description
Left: Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at 1:500 dilution.

Below: Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 µg/ml.

Other Product Images

Source : GFR alpha 1 antibody was raised against a peptide corresponding to amino acids 369 to 382 of human GFR alpha 1.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : GFR alpha 1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human GFR alpha 1 (Intermediate Domain) Peptide (Cat. No. 1133P)
Application : GFR alpha 1 antibody can be used for detection of GFR alpha 1 by Western blot. The amino acid sequence of immunogenic peptide is identical to those of mouse and rat and the antibody recognizes GFR alpha 1 from human, mouse and rat origins.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1133P - GFR alpha 1 Peptide
Long-Term Storage : GFR alpha 1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1303 - Human Brain Tissue Lysate
2. Cat. No. 1304 - Human Liver Tissue Lysate
3. Cat. No. 1305 - Human Kidney Tissue Lysate
4. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H, M, R
GI Number : 20141405
Accession Number : P56159
Short Description : (IN) GDNF receptor
References

1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z; Cupples R; Louis JC; Hu S; Altrock BW; Fox GM. GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR-a, a novel receptor for GDNF. Cell, 1996;85:1113-24.
2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD; Gray C; Armanini MP; Pollock RA; Herti F; Phillips HS; Goddard A; Moore MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF. Nature, 1996;382:80-83.
3. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L, Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci U S. 1997;94:6238-6243
4. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A, Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724