DDX3 Antibody

DDX3 Antibody

Catalog# : 3759

DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases (1,2). DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome (3). DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation (4). It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma (5). Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm (6).
Additional Names : DDX3 (IN), Dead box polypeptide 3 X-linked, DBX, Helicase-like protein 2
Description
Left: Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 µg/ml.

Other Product Images

Source : DDX3 antibody was raised against a 16 amino acid peptide from near the center of human DDX3.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : DDX3 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human DDX3 (Intermediate Domain) Peptide (Cat. No. 3759P)
Application : DDX3 antibody can be used for the detection of DDX3 by Western blot at 1 – 2 µg/ml.DDX3 antibody will detect both DDX3 and DBY.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3759P - DDX3 Peptide
Long-Term Storage : DDX3 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity :H, M, R
GI Number : 3523150
Accession Number : AAC34298
Short Description : (IN) An RNA helicase that may be involved in HIV-1 nuclear export
References
1. Linder P, Lasko PF, Ashburner M, et al. Birth of the D-E-A-D box. Nature 1989; 337:121-2.
2. Park SH, Lee SG, Kim Y, et al. Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3-->p11.23. Cytogenet. Cell Genet. 1998; 81:178-9.
3. Lahn BT and Page DC. Functional coherence of the human Y chromosome. Science 1997; 278:675-80.
4. Abdelhaleem M. RNA helicases: regulators of differentiation. Clin. Biochem. 2005; 38:499-503