Bora Antibody

Bora Antibody

Catalog# : 5117

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.
Additional Names : Bora (NT), Protein aurora borealis, C13orf34, Chromosome 13 open reading frame 34

Description
Left: Western blot analysis of Bora in Jurkat cell lysate with Bora antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of Bora in mouse brain tissue with Bora antibody at 2.5 µg/ml.

Other Product Images

Source : Bora antibody was raised against a 15 amino acid peptide from near the amino terminus of human Bora.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Bora antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Bora antibody can be used for detection of Bora by Western blot at 1-2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5117P - Bora Peptide
Long-Term Storage : Bora antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity :H, M, R
GI Number : 74737659
Accession Number : Q6PGQ7
Short Description : (NT) Protein aurora borealis
References
1. Berdnik D and Knoblich JA. Drosophila Aurora A is required for centrosome maturation and Actin-dependent asymmetric protein localization during mitosis. Curr. Biol. 2002; 12:640-647.
2. Wiese C and O’Brien LL. What's so Bor(a)ing about Aurora A activation? Dev. Cell 2006; 11:133-134.
3. Hutterer A, Berdnik D, Wirtz-Peitz F, et al. Mitotic activation of the kinase Aurora A requires its binding partner Bora. Dev. Cell 2006; 11:147-157.
4. Fu J, Bian M, Jiang Q, et al.. Roles of Aurora kinases in mitosis and tumorigenesis. Mol. Cancer Res. 2007; 5:1-10.