ATM Antibody

ATM Antibody

Catalog# : 3945

DNA double strand breaks represent a major threat to an organism’s genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.
Additional Names : ATM, mutated in Ataxia-Telangiectasia
Description
Left: Western blot analysis of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of ATM in human lymph node tissue with ATM antibody at 2.5 µg/ml.

Other Product Images

Source : ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ATM antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : ATM antibody can be used for detection of ATM by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3945P - ATM Peptide
Long-Term Storage : ATM antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1224 - Daudi Cell Lysate
2. Cat. No. 1369 - Human Lymph node Tissue Lysate
Species Reactivity :H
GI Number : 2304971
Accession Number : AAB65827
Short Description : DNA double-stranded break signaling molecule
References

1. Cahill D, Connor B, and Carney JP. Mechanisms of eukaryotic DNA double strand break repair. Front. Biosci. 2006 ; 11 :1958-76.
2. 2. Dasika GK, Lis SC, Zhao S, et al. DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis. Oncogene 1999; 18:7883-99.