MED4 Antibody

MED4 Antibody

Catalog# : 4729

The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED4. This protein has also been shown to be part of the vitamin D receptor interacting complex. MED4 was recently identified as an HIV dependency factor (HDF), suggesting that MED4 may be an important drug target in HIV treatment. At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
Additional Names : MED4, Mediator of RNA polymerase II transcription subunit 4, vitamin d receptor interacting protein, vdrip, drip36, trap36
Description
Left: Western blot analysis of MED4 in human testis tissue lysate with MED4 antibody at 0.5 µg/ml.

Below: Immunohistochemistry of MED4 in human testis tissue with MED4 antibody at 10 μg/ml.




Source : MED4 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of human MED4.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MED4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : MED4 antibody can be used for detection of MED4 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4729P - MED4 Peptide
Long-Term Storage : MED4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity :H, M, R
GI Number : 7661788
Accession Number : NP_054885
Short Description : Mediator of RNA polymerase II transcription subunit 4
References
1. Sato S, Tomomori-Sato C, Parmely TJ, et al. A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Mol. Cell 2004; 14:685-91.
2. Gustafsson CM, Myers LC, Beve J, et al. Identification of new mediator subunits in the RNA polymerase II holoenzyme from Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:30851-4.
3. Rachez C, Lemon BD, Suldan Z, et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature 1999; 398:824-8.
4. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.