APOBEC3G Antibody

APOBEC3G Antibody

Catalog# : 3257

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5alpha which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3) as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome (4,5). This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity (6).

Additional Names : APOBEC3G (NT), APOBEC3G

Description
Left: Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 µg/ml.

Below: Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 µg/ml.

Other Product Images
Source : APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G APOBEC3G antibody will also detect the APOBEC3F isoform.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : APOBEC3G antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human APOBEC3G (N-Terminus) Peptide (Cat. No. 3257P)
Application : APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3257P - APOBEC3G Peptide
Long-Term Storage : APOBEC3G antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1223 - Caco-2 Cell Lysate
2. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 13399304
Accession Number : NP_068594
Short Description : (NT) an HIV restriction factor
References

1. Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
2. Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
3. Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
4. Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.