Wednesday, June 1, 2011

ACVR1B Antibody


Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I and two type II receptors. ACVR1B, also known as activin receptor-like kinase 4 (ALK4), is a type I receptor for activin and plays major roles in cell differentiation, growth arrest and apoptosis. Like another type I activin receptor ACVR1C, ACVR1B can mediate signaling by ligands such as Nodal, Xnr1, GDF-1/3, activin B and activin AB. In Xenopus embryos, expression of a dominant-negative form of ACVR1B blocked all mesoderm-inducing ligands, while expression of a dominant negative ACVR1C only blocked Nodal and Xnr1 signaling, suggesting that the ACVR1B and ACVR1C possess distinct functions. At least three isoforms of ACVR1B are known to exist. This antibody is predicted to have no cross-reactivity to ACVR1 or ACVR1C.

Additional Names: ACVR1B, Activin A receptor type IB, Activin receptor-like kinase 4, Activin receptor type 1B precursor, ACTR-IB, ACVRLK4, ALK4, ALK-4


Left: Western blot analysis of ACVR1B in human kidney tissue lysate with ACVR1B antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

ACVR1B antibody was raised against a 14 amino acid peptide near the carboxy terminus of the human ACVR1B.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: ACVR1B antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: ACVR1B antibody can be used for detection of ACVR1B by Western blot at 1 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4793P - ACVR1B Peptide
Long-Term Storage: ACVR1B antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity: H, M
GI Number: 4501895
Accession Number: NP_001096
Short Description: Activin A receptor type IB
1. Tsuchida K, Sawchenko PE, Nishikawa S, et al. Molecular cloning of a novel type I receptor serine/threonine kinase for the TGF beta superfamily from rat brain. Mol. Cell. Neurosci. 1996; 7:467–78.
2. Reissmann E, Jornvall H, Blokzijl A, et al. The orphan receptor ALK7 and the activin receptor ALK4 mediate signaling by nodal proteins during vertebrate development. Genes Dev. 2001; 15:2010–22.
3. Tsuchida K, Nakatani M, Yamakawa N, et al. Activin isoforms signal through type I receptor serine/threonine kinase ALK7. Mol. Cell Endocrinol. 2004; 220:59-65.

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