Thursday, June 30, 2011
DNA Sequence Analysis
For over 20 years, BioSynthesis has provided custom oligonucleotide production services worldwide, including researcher at university, biotechnology and pharmaceutical institutions. We offer wide variety of oligo modifications and labelings from small discovery research scale to multi-gram ASR/cGMP for clinical diagnostic production. We offer design and synthesis of oligonucleotide primers and probes, also provides full range of DNA microarray printing, processing and analysis.
The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide bases, adenine, guanine, cytosine, and thymine, in a DNA oligonucleotide. The sequence of DNA constitutes the heritable genetic information in nuclei, plasmids, mitochondria, and chloroplasts that forms the basis for the developmental programs of all living organisms.
While the chemical sequencing method of Maxam and Gilbert, and the plus-minus method of Sanger and Coulson were orders of magnitude faster than previous methods, the chain-terminator method developed by Sanger was even more efficient, and rapidly became the method of choice. The Maxam-Gilbert technique requires the use of highly toxic chemicals, and large amounts of radiolabeled DNA, whereas the chain-terminator method uses fewer toxic chemicals and lower amounts of radioactivity.
Recombinant DNA is a man-made DNA sequence that has been assembled from other DNA sequences. They can be transformed into organisms in the form of plasmids or in the appropriate format, by using a viral vector.