Friday, June 17, 2011

GAPDH Antibody


Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer’s and Huntington’s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Additional Names: GAPDH, Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD


Western blot analysis of GAPDH in HeLa cell lysate with GAPDH antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 µg/ml.

Other Product Images
Source:GAPDH antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human GAPDH.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: GAPDH antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human GAPDH / Glyceraldehyde-3-phosphate dehydrogenase / G3PDH / GAPD (C-Terminus) Peptide (Cat. No.
Application: GAPDH antibody can be used for the detection of GAPDH by Western blot at 0.5 – 1 µg/ml.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3781P - GAPDH Peptide
Long-Term Storage: GAPDH antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1.Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity: H, M, R
GI Number: 120649
Accession Number: P04406
Short Description: (CT) an enzyme in the glycolytic pathway
1. Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
2. Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.

No comments: