Wednesday, June 15, 2011


DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist.

Additional Names: DARC (CT), Duffy antigen, Fy glycoprotein, GpFy, Cell adhesion molecule 3, CADM3
Western blot analysis of DARC in mouse brain tissue lysate with DARC antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below: Immunohistochemistry of DARC in mouse brain tissue with DARC antibody at 2.5 µg/ml.

Other Product Images
Source:DARC antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human DARC.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: DARC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: DARC antibody can be used for detection of DARC by Western blot at 1 – 2 µg/ml.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4069P - DARC Peptide
Long-Term Storage: DARC antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity: H, M, R
GI Number: 67476970
Accession Number: Q16570
Short Description: (CT) Duffy antigen/chemokine receptor

1. Chaudhuri A, Polyakova J, Zbrzezna V, et al. Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. Proc. Natl. Acad. Sci. USA 1993; 90:10793-7.
2. Gardner L, Patterson AM, Ashton BA, et al. The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun. 2004; 321:306-12.
3. Gil ML, Vita N, Lebel-Binay S, et al. A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol. 1992; 2826-33.

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