Wednesday, June 22, 2011

IKK beta Antibody


Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, and bacteria product LPS. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IkappaB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKbeta). IKKalpha and IKKbeta interact with each other and both are essential for NF-kappaB activation. IKKbeta phosphorylates both IkappaB-alpha and IkappaB-beta. IKKbeta is expressed in variety of human tissues.

Additional Names: IKK beta (C3), IKK beta, I-kappa-B-kinase beta, I-kappa-B-kinase 2


Western blot analysis of IKK beta in Jurkat whole cell lysate with IKK beta antibody (C3) at 1:500 dilution.

Below: Immunocytochemistry staining of HeLa cells using IKK beta antibody at 10 µg/ml.

Other Product Images
Source:IKK beta antibody was raised against a peptide corresponding to amino acids near the carboxy-terminus of human IKK beta (Genbank accession NoO14920), which differs from corresponding murine sequence by one amino acid.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IKK beta antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Immunogen: Human IKK beta (C-Terminus 3) Peptide (Cat. No. 2121P)
Application: IKK beta antibody can be used for detection of IKK beta by Western blot at 1 – 2 µg/ml.A 87 kDa band should be detected. This polyclonal antibody has no cross response to IKKa or IKKy.
Tested Application(s): E, WB, ICC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 2121P - IKK beta Peptide
Long-Term Storage: IKK beta antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1201 - HeLa Whole Cell Lysate
Species Reactivity: H
GI Number: 14285497
Accession Number: O14920
Short Description: (C3) Ikappa B kinase beta

1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997;388:548-54
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IkappaB kinase. Cell 1997;90:373-83
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997;91:243-52
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-alpha: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9

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