Wednesday, June 22, 2011

IKK epsilon Antibody


Catalog#:2329

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. IkB is phosphorylated by IkB kinase (IKK) complex that contains IKKalpha, IKKbeta, and IKKgamma. A novel molecule in the IKK complex was recently identified and designated IKKepsilon/IKK-i (1,2). IKK epsilon is required for the activation of NF-kappaB by PMA and T cell receptors but not by TNFalpha and IL-1 (1). IKKepsilon/IKK-i message is expressed in a variety of tissues and is inducible by TNFalpha, IL-1, and LPS (2).

Additional Names: IKK epsilon (CT), IKKi, IKKe

Description

Left:
Western blot analysis of IKK epsilon in Jurkat whole cell lysate with IKK epsilon/IKK-i antibody at 1 µg/ml.

Below: Immunohistochemistry of IKK epsilon in human pancreas tissue with IKK epsilon antibody at 10 µg/ml.

Other Product Images
Source:IKK epsilon antibody was raised against a peptide corresponding to amino acids 701 to 716 of human IKK epsilon/IKK-i.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IKK epsilon antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human IKK epsilon Peptide (Cat. No. 2329P)
Application: IKK epsilon antibody can be used for detection of IKK epsilon by Western blot at 0.5 to 1 µg/ml.An 80 kDa band should be detected. It has no cross response to IKKa, IKKb, or IKKy.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 2329P - IKK epsilon Peptide
Long-Term Storage: IKK epsilon antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1205 - Jurkat Cell Lysate
2. Cat. No. 1307 - Human Pancreas Tissue Lysate
Species Reactivity: H
GI Number: 7288877
Accession Number: AF241789
Short Description: (CT) IkB Kinase epsilon
References

1. Peters RT, Liao SM, Maniatis T. IKK epsilonpsilon is part of a novel PMA-inducible IkappaB kinase complex. Mol Cell 2000;5(3):513-22
2. Shimada T, Kawai T, Takeda K, Matsumoto M, Inoue J, Tatsumi Y, Kanamaru A, Akira S. IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int Immunol 1999;11(8):1357-62 (WD010

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