Thursday, June 16, 2011

EphA10 Antibody


Eph receptors, the largest subfamily of receptor tyrosine kinases (RTKs), and their ephrin ligands are important mediators of cell–cell communication regulating cell attachment, shape, and mobility of neuronal and endothelial cells in central nervous system function and in development. Eph receptors can be divided into two subgroups: EphA and EphB. In mammals, the EphA class consists of eight members (EphA 1-7 and 10) that in general bind to ephrin-A members linked to the cell membrane through a glycosylphosphatidylinositol linkage. The EphB class consists of six members (EphB 1-6) that in general bind ephrin-B members that transverse the cell membrane. The Ephrin / EPH signaling pathway networks with the WNT signaling pathway during embryogenesis, tissue regeneration, and carcinogenesis. Recent studies show that Eph/EFN might be relevant in normal B-cell biology and could represent new potential prognostic markers and therapeutic targets for CLL.

Additional Names: EphA10, EPH receptor A10, Ephrin type-A receptor 10, FLJ16103


Left: Western blot analysis of EphA10 in 293 cell lysate with EphA10 antibody at (A) 1 µg/ml and (B) 2 µg/ml.

Source:EphA10 antibody was raised against a 14 amino acid peptide of near the amino terminus of human EphA10.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: EphA10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: EphA10 antibody can be used for detection of EphA10 by Western blot at 1 - 2 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 5253P - EphA10 Peptide
Long-Term Storage: EphA10 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1210 - 293 Cell Lysate
Species Reactivity: H, M, R
GI Number: 150456460
Accession Number: NP_001092909
Short Description: EPH receptor A10
1. Flanagan JG and Vanderhaeghen P. The ephrins and Eph receptors in neural development. Annu. Rev. Neurosci. 1998;.21:309–45.
2. Frisen J, Holmberg J, and Barbacid M. Ephrins and their Eph receptors: multitalented directors of embryonic development. EMBO J. 1999; 18:5159–65.
3. Eph Nomenclature Committee. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Cell 1997; 90:403–4.
4. Holder N and Klein R. Eph receptors and ephrins: effectors of morphogenesis, Development 1999; 126:2033–44.

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