Catalog#:3749
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2 . It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32 (2). IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-alpha, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNgamma, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis (3).
Additional Names: IL-32 (CT), Interelukin 32
Description
Left: Western blot analysis of IL-32 in human spleen cell lysate with IL-32 antibody at (A) 5 and (B) 10 µg/ml shows two isoforms of IL-32. Below: Immunohistochemistry of IL-32 in human spleen tissue with IL-32 antibody at 10 µg/ml.
Source:IL-32 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human IL-32.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IL-32 antibody was raised in rabbit.
Immunogen: Human IL-32 (C-Terminus) Peptide (Cat. No. 3749P)
Application: IL-32 antibody can be used for the detection of IL-32 by Western blot at 5 – 10 µg/ml.This antibody detects all isoforms of IL-32.
Tested Application(s): E, WB, IHC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3749P - IL-32 Peptide
Long-Term Storage: IL-32 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity: H, M
GI Number: 14424787
Accession Number: AAH09401
Short Description: (CT) Interleukin 32
References
1. Dahl CA, Schall RP, He HL, et al. Identification of a novel gene expressed in activated natural killer cells and T cells. J. Immunol. 1992; 148:597-603.
2. Kim S-H, Han S-Y, Azam T, et al. Interleukin-32: a cytokine and inducer of TNF-a. Immunity 2005; 22:131-42.
3. Cagnard N, Letourneur F, Essabbani A, et al. Interleukin-32, CCL2, PF4F1 and GFD10 are the only cytokine/chemokine genes differentially expressed by in vitro cultured rheumatoid and osteoarthritis fibroblast-like synoviocytes. Eur. Cyto. Network 2005; 16:289-92.
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