Thursday, June 30, 2011

IL-31 Antibody

IL-31 Antibody


Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells (1,2). IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor (2). In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways (3). RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells . IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia, and skin lesions and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development (1,4).

Additional Names: IL-31 (IN), Interleukin 31, IL31


Left: Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 µg/ml.

Source:IL-31 antibody was raised against a 18 amino acid peptide from near the center of human IL-31.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IL-31 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human IL-31 (Intermediate Domain) Peptide (Cat. No. 3747P)
Application: IL-31 antibody can be used for the detection of IL-31 by Western blot at 2.5 – 5 µg/ml.Despite its predicted size, IL-31 runs at approximately 27 - 30 kDa in SDS-PAGE.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 3747P - IL-31 Peptide
Long-Term Storage: IL-31 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Species Reactivity: H, M
GI Number: 46241139
Accession Number: AAS82846
Short Description: (IN) Interleukin 31
1. Dillon SR, Sprecher C, Hammond A, et al. Interleukin 31, a cytokine produced by activated T cells, induces dermatitis in mice. Nat. Immunol. 2004; 5:752-60.
2. Dreuw A, Radtke S, Pflanz S, et al. Characterization of the signaling capacities of the novel gp130-like cytokine receptor. J. Biol. Chem. 2004; 279:36112-20.
3. Diveu C, Lak-Hal AH, Froger J, et al. Predominant expression of the long isoform of GP130-like (GPL) receptor is required for interleukin-31 signaling. Eur. Cytokine Netw. 2004; 15:291-302.
4. Bilsborough J, Leung DY, Maurer M, et al. IL-31 is associated with cutaneous lymphocyte antigen-positive skin homing T cells in patients with atopic dermatitis. J. Allergy Clin. Immunol. 2006; 117:418-25.

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