Tuesday, June 14, 2011
For over 20 years, BioSynthesis has provided custom oligonucleotide production services worldwide, including researcher at university, biotechnology and pharmaceutical institutions. We are not only synthesizing standard DNA primer and probes but are also specialized in production and purification of modified oligomers. Base, ribose and backbone modifications and reporter groups like dyes and haptens are routinely incorporated at small discovery research scale to multi-gram ASR/cGMP for clinical diagnostic production. As always, quality is guaranteed!
An oligonucleotide (or oligo) is a short segment of RNA or DNA, typically with twenty or fewer bases. Although they can be formed by cleavage of longer segments, they are now more commonly synthesized by polymerizing individual nucleotide precursors. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. The length of the oligonucleotide is usually denoted by "mer" (from Greek meros, "part"). For example, a fragment of 25 bases would be called a 25-mer. Oligonucleotides are often used as probes for detecting DNA or RNA because they bind readily to their complements.
Oligonucleotides are chemically synthesized using nucleotides, called phosphoramidites, normal nucleotides that have protection groups: preventing amine, hydroxyl groups and phosphate groups interacting incorrectly. One phosphoramidite is added at the time, the product's 5' phosphate is deprotected, and a new base is added, and so on (backwards); at the end, all the protection groups are removed.
Oligonucleotide synthesis is the non-biological, chemical synthesis of defined short sequences of nucleic acids. It is extremely useful in laboratory procedures covering a wide range of molecular biology applications. Automated synthesizers allow the synthesis of oligonucleotides up to 160 to 200 bases. Typically, synthesized oligonucleotides are single-stranded DNA molecules around 15-20 bases in length.!