Tuesday, June 21, 2011

IKK alpha Antibody


Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and bacteria product LPS. NF-kappaB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. The long-sought IkappaB kinase (IKK), which phosphorylates IkappaB, and mediates IkappaB degradation and NF-kappaB activation, was recently identified by several laboratories (1-5). IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKalpha and IKKΒ). IKKalpha and IKKb interact with each other and both are essential for the NF-kappaB activation. IKKalpha specifically phosphorylates IkappaB-Α.IKKalpha is expressed in a variety of human tissues.

Additional Names: IKK alpha (C2), Inhibitor of nuclear factor kappa-B kinase alpha subunit, IkappaB kinase alpha, NFkappaB inhibitor kinase alpha, IKKa


Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.

Below: Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 µg/ml.

Other Product Images
Source:IKK alpha antibody was raised against a peptide corresponding to amino acids 699 to 715 of human IKK alpha, which differs from corresponding murine sequence by one amino acid
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IKK alpha antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen: Human IKK alpha (C-Terminus 2) Peptide (Cat. No. 2115P)
Application: IKK alpha antibody can be used for detection of IKK alpha by Western blot at 1:500 to 1:1000 dilution. A 85 kDa band should be detected. This polyclonal antibody has no cross response to IKKb or IKKy.
Tested Application(s): E, WB, ICC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat. No. 2115P - IKK alpha Peptide
Long-Term Storage: IKK alpha antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity: H
GI Number: 2327068
Accession Number: AF009225
Short Description: (C2) Ikappa B kinase alpha
1. DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature 1997;388:548-54
2. Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an IkappaB kinase. Cell 1997;90:373-83
3. Zandi E, Rothwarf DM, Delhase M, Hayakawa M, Karin M. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKalpha, necessary for IkappaB phosphorylation and NF-kappaB activation. Cell 1997;91:243-52
4. Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DY. IkappaB kinase-beta: NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK. Science 1997;278:866-9

No comments: