Tuesday, June 21, 2011

IKAP Antibody


IKAP was initially identified as a scaffold protein of the IkappaB kinase complex that could bind to IKKalpha, IKKbeta, NF-kappaB, and the NF-kappaB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.

Additional Names: IKAP, IKK complex-associated protein, IKBKAP


Western blot analysis of IKAP in A-20 cell lysate with IKAP antibody at in (A) 0.5, and (B) 1 µg/ml.

Below: Immunocytochemistry of IKAP in A-20 cells with IKAP antibody at 1 µg/ml.

Other Product Images
Source:IKAP antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human IKAP.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: IKAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: IKAP antibody can be used for detection of IKAP by Western blot at 0.5 to 1 µg/ml.
Tested Application(s): E, WB, ICC
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 2337P - IKAP Peptide
Long-Term Storage: IKAP antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1288 - A20 Cell Lysate
Species Reactivity: H, M
GI Number: 3757822
Accession Number: AAC64258
Short Description: part of the RNA polymerase II transcription elongation complex
1. Cohen L, Henzel WJ, and Baeuerle PA. IKAP is a scaffold protein of the IkB kinase complex. Nature 1998; 395:292-6.
2. Krappmann D, Hatada EN, Tegethoff S, et al. The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component. J. Biol. Chem. 2000; 275:29779-87.
3. Anderson SL, Coli R, Daly IW, et al. Familial dysautonomia is caused by mutations of the IKAP gene. Am. J. Hum. Genet. 2001; 68:753-8.
4. Hawkes NA, Otero G, Winkler GS, et al. Purification and characterization of the human elongator complex. J. Biol. Chem. 2002; 277:3047-52.

1 comment:

Cell Death Research said...

Hello Dude,

It is a human gene that provides instructions to make the IKAP protein, which is found in a variety of cells throughout the body, including brain cells. Although the exact function of the IKAP protein is not clearly understood, it probably plays a role in transcription. Thanks for sharing it......