Thursday, June 16, 2011

Disp2 Antibody


Disp2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the Disp proteins have been implicated in these pathways. Recently, it has been shown that Disp2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased Disp2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by Disp2 mutants. At least two isoforms of Disp2 are known to exist. This Disp2 antibody is predicted to not cross-react with Disp1 or Disp3.

Additional Names: Disp2 (CT), Dispatched homolog 2, DISPB


Left: Western blot analysis of Disp2 in rat brain tissue lysate with Disp2 antibody at (A) 1 and (B) 2 µg/ml.

Source:Disp2 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human Disp2.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: Disp2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application: Disp2 antibody can be used for the detection of Disp2 by Western blot at 1 – 2 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4883P - Disp2 Peptide
Long-Term Storage: Disp2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1. Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity: H, M, R
GI Number: 160380692
Accession Number: A7MBM2
Short Description: (CT) Dispatched homolog 2

1. Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6.
2. Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther. 2005; 4:1050-4.
3. Li N, Flynt AS, Kim HR, et al. Dispatched homolog 2 is targeted by miR-214 through a combination of three week microRNA recognition sites. Nuc. Acids Res. 2008; 36:4277-85.

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