Monday, June 20, 2011

GDF6 Antibody


Growth differentiation factors (GDFs) are members of the transforming growth factor (TGF) superfamily that is involved in embryonic development and adult tissue homeostasis. Both GDF6 and GDF7 are closely related to GDF5 which has been shown to induce activation of plasminogen activator, thereby inducing angiogenesis. It is predominantly expressed in long bones during fetal embryonic development and is involved in bone formation. In Xenopus, GDF6 is expressed at the edge of the neural plate and within the anterior neural plate including the eye fields. GDF6 is required for normal formation of some bones and joints in the limbs, skull, and axial skeleton. It may regulate patterning of the ectoderm by interacting with bone morphogenetic proteins (BMPs), and control eye development. Mutations in this gene result in colobomata, which are congenital abnormalities in ocular development, and in Klippel-Feil syndrome (KFS), a congenital disorder of spinal segmentation.

Additional Names: GDF6 (CT), Growth Differentiation Factor 6, GDF16, GDF-6, CDMP2


Left: Western blot analysis of GDF6 in SK-N-SH lysate with GDF6 antibody at (A) 0.5 and (B) 1 µg/ml.

Source:GDF6 antibody was raised against a 17 amino acid peptide near the carboxy terminus of the human GDF6.
Purification: Affinity chromatography purified via peptide column
Clonality and Clone: This is a polyclonal antibody.
Host: GDF6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application: GDF6 antibody can be used for detection of GDF6 by Western blot at 1 µg/ml.
Tested Application(s): E, WB
Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide:Cat.No. 4691P - GDF6 Peptide
Long-Term Storage: GDF6 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control:
1.Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity: H, M, R
GI Number: 48475062
Accession Number: NP_001001557
Short Description: (CT) Growth Differentiation Factor 6
1. Massague J. 1990. The transforming growth factor-beta family. Ann. Rev. Cell Biol. 6:597-641.
2. McPherron AC, Lawler AM, and Lee SJ. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature 1997; 387:83-90.
3. Hanel ML and Hensey C. Eye and neural defects associated with loss of GDF6. BMC Dev. Biol. 2006; 6:43.
4. Settle SH Jr., Rountree RB, Sinha A, et al. Multiple joint and skeletal patterning defects caused by single and double mutations in the mouse Gdf6 and Gdf5 genes. Dev. Biol. 2003; 254:116-130.

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