Friday, March 25, 2011

LGP2 Antibody

LGP2 Antibody

Catalog# :4353

Anti-viral innate immune responses are triggered by pathogen-associated molecular patters (such as the accumulation of intracellular nucleic acids resulting from virus infections) and represent the first line of defense against numerous infectious organisms. The Toll-like receptors (TLR) 3, 7, 8, and 9 are expressed in immune cells and function as transmembrane pattern recognition receptors to detect foreign nucleic acids. Other proteins that play similar roles, such as RIG-1 and MDA5 are members of a CARD-helicase family and are expressed in the cytoplasm. A third protein, LGP2, is similar to RIG-1 and MDA5, except for lacking a homologous CARD domain. It is thought to act as an element of negative-feedback regulation of intracellular antiviral signaling. At least four isoforms of LGP2 are known to exist.

Additional Names : LGP2 (NT), DHX58, likely ortholog of mouse D11lgp2

Left: Western blot analysis of LGP2 in MDA-MB-361 cell lysate with LGP2 antibody at (A) 0.5 and (B) 1 µg/ml.

Source :LGP2 antibody was raised against a 14 amino acid peptide from near the amino terminus of human LGP2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : LGP2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : LGP2 antibody can be used for detection of LGP2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4353P - LGP2 Peptide
Long-Term Storage : LGP2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1217 - MDA-MB-361 Cell Lysate
Species Reactivity : H, M, R
GI Number : 119581202
Accession Number : EAW60798
Short Description : (NT) DHX58
1. Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
2. Iwasaki A and Medzhitov R. Toll-like receptor control of the adaptive immune responses. Nat. Immunol. 2004; 5:987-95.
3. Yoneyama M, Kikuchi K, Matsumoto K, et al. The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses. Nat. Immunol. 2004; 5:730-7.
4. Andrejeva J, Childs KS, Young DF, et al. The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibits its activation of the IFN-betta promoter. Proc. Natl. Acad. Sci. USA, 2004; 101:17264-9.

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