Friday, March 25, 2011

MD-2 Monoclonal Antibody

MD-2 Monoclonal Antibody

Catalog# :PM-4845

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.

Additional Names : MD-2 (9F1B1): Lymphocyte antigen 96 precursor, ESOP-1
Left: Western blot analysis of (A) 25 and (B) 125 ng of MD-2 recombinant protein with MD-2 antibody at 1 µg/ml. Below: Immunohistochemistry of MD-2 in human spleen with MD-2 antibody at 2.5 µg/ml.

Source :Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2
Purification : Immunoaffinity chromotography purified IgG
Clonality and Clone : This is a monoclonal antibody. (Clone 9F1B1)
Host : MD-2 monoclonal antibody was raised in mouse.
Immunogen : Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2
Application : MD-2 antibody can be used for detection of MD-2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :
Long-Term Storage : MD-2 monoclonal antibody can be stored at 4ºC, stable for one year.
Positive Control :
1. Cat. No. 95-108 - MD-2 Recombinant Protein
Species Reactivity : H, M
Short Description : Co-receptor with TLR4 for LPS
1. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
2. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
3. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
4. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.

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