Catalog# : 4727
The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.
Additional Names : POLR3F, RNA polymerase III polypeptide F, RNA polymerase subunit C6, RPC6, RPC39
Left: Western blot analysis of POLR3F in human brain tissue lysate with POLR3F antibody at (A) 0.5 and (B) 1 µg/ml.
Source :POLR3F antibody was raised against a 21 amino acid peptide from near the amino terminus of human POLR3F.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : POLR3F antibody was raised in rabbit.
Application : POLR3F antibody can be used for detection of POLR3F by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4727P - POLR3F Peptide
Long-Term Storage : POLR3F antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 10636565
Accession Number : CAC11110
Short Description : RNA polymerase III polypeptide F
1. Hu P, Wu S, Sun Y, et al. Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. Mol. Cell Biol. 2002; 22:8044-55.
2. Haeusler RA and Engelke DR. Spatial organization of transcription by RNA polymerase III. Nucleic Acids Res. 2006; 34:4826-36. Epub 2006 Sep 13.
3. Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science 2008; 319:921-6.