Wednesday, March 9, 2011

STIM2 Antibody

STIM2 Antibody

Catalog# :4125

In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.

Additional Names : STIM2 (IN), Stromal interaction molecule 2
Below: Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.

Below:Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 µg/ml.

Other Product Images
Source :STIM2 antibody was raised against a 17 amino acid peptide from near the center of human STIM2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : STIM2 antibody was raised in rabbit.
Application : STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4125P - STIM2 Peptide
Long-Term Storage : STIM2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1288 - A20 Cell Lysate
2. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity : H, M, R
GI Number : 41349446
Accession Number : NP_065911
Short Description : (IN) an inhibitor of STIM1
1. Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells. Trends Mol. Med. 2007; 13:103-7.
2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
3. Zhang SL, Yu Y, Roos J, et al. STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane. Nature 2005; 437:902-5.
4. Spassova MA, Soboloff J, He L-P, et al. STIM1 has a plasma membrane role in the activation of store-operated Ca2+ channels. Proc. Natl. Acad. Sci. USA 2006; 103:4040-5.

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