Friday, March 18, 2011

Seasonal H1N1 Nucleocapsid Protein Antibody

Seasonal H1N1 Nucleocapsid Protein Antibody

Catalog# :5361

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus (S-OIV) was identified in specimens obtained from patients in Mexico and the United States. The influenza A virus polymerase transcribes and replicates eight virion RNA (vRNA) segments, among which the nucleocapsid protein (NP), thought to control whether mRNA or cRNA is produced. The nucleoprotein (NP), which has multiple functions during the virus life cycle, possesses regions that are highly conserved among influenza A, B, and C viruses. It was recently found several NP mutations that affected the efficient incorporation of multiple viral-RNA (vRNA) segments into progeny virions even though a single vRNA segment was incorporated efficiently. This indicates that the respective conserved amino acids in NP may be critical for the assembly and/or incorporation of sets of eight vRNA segments. This antibody is specific for the seasonal H1N1 influenza NP and will not recognize the corresponding NP sequence from the swine-origin H1N1 influenza (A/California/14/2009 (H1N1)).

Additional Names : Seasonal Influenza A (H1N1) Nucleocapsid Protein, NP, Common flu NP
Left: ELISA results using Seasonal H1N1 Nucleocapsid Protein antibody at 1 µg/ml and the blocking and corresponding peptides at 60, 10, 2 and 0 ng/ml.
Source :NP antibody was raised against a synthetic peptide from the Seasonal H1N1 NP protein.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Seasonal H1N1 Nucleocapsid Protein antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : NP antibody can be used for the detection of the NP protein from the H1N1 strain of Seasonal Influenza A in ELISA.
Tested Application(s) : E
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5361P - Seasonal H1N1 Nucleocapsid Protein Peptide
Long-Term Storage : Seasonal H1N1 Nucleocapsid Protein antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity : V
GI Number : 260593857
Accession Number : ACX46223
Short Description : Influenza Nucleocapsid Protein
1. Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.
2. Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team, Dawood FS, Jain S, et al. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N. Engl. J. Med. 2009; 360:2605-15.
3. Li Z, Watanabe T, Hatta M, et al. Mutational analysis of conserved amino acids in the influenza A virus nucleoprotein. J. Virol. 2009; 83:4153-62.
4. Newcomb LL, Kuo RL, Ye Q, et al. Interaction of the influenza a virus nucleocapsid protein with the viral RNA polymerase potentiates unprimed viral RNA replication. J. Virol. 2009; 83:29-36.

No comments: