Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
Additional Names : ORAI2, Transmembrane protein 142B, TMEM142B, Calcium release-activated calcium channel protein 2
Left: Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 µg/ml.
Below: Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 µg/ml.
Other Product Images
Source :ORAI2 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human ORAI2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ORAI2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4111P - ORAI2 Peptide
Long-Term Storage : ORAI2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity : H, M
GI Number : 74732728
Accession Number : Q96SN7
Short Description : (CT) a T cell calcium channel
1. Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
3. Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
4. Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90.