Monday, March 21, 2011

RNA Polymerase

For over 20 years, Bio Synthesis has provided custom oligonucleotide production services worldwide, including researcher at university, biotechnology and pharmaceutical institutions. We offer wide variety of oligo modifications such as RNA syntehsis from small discovery research scale to multi-gram ASR/cGMP for clinical diagnostic production.

RNA Polymerase

RNA polymerase (RNAP or RNApol) is an enzyme that makes an RNA copy of a DNA or RNA template. In cells, RNAP is needed for constructing RNA chains from DNA genes, a process called transcription. RNA polymerase enzymes are essential to life and are found in all organisms and many viruses. In chemical terms, RNAP is a nucleotidyl transferase that polymerizes ribonucleotides at the 3' end of an RNA transcript.

RNA Polymerase In Archaea

Archaea have a single RNAP that is closely related to the three main eukaryotic polymerases. Thus, it has been speculated that the archaeal polymerase resembles the ancestor of the specialized eukaryotic polymerases.

RNA Protection Assays

A nuclease protection assay is a laboratory test used in biochemistry and genetics to identify individual RNA molecules in a heterogeneous RNA sample extracted from cells. The technique can identify one or more RNA molecules of known sequence even at low total concentration.

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