The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.
Additional Names : MDA5 (IN), Melanoma differentiation-associated protein 5, Interferon-induced with helicase C domain protein 1, IFIH1
Left: Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 µg/ml. Below: Immunohistochemistry of MDA5 in human lymph node tissue with MDA5 antibody at 5 µg/ml.
Source :MDA5 antibody was raised against a 16 amino acid peptide from near the center of human MDA5.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MDA5 antibody was raised in rabbit.
Application : MDA5 antibody can be used for detection of MDA5 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4039P - MDA5 Peptide
Long-Term Storage : MDA5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1224 - Daudi Cell Lysate
2. Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity : H, M
GI Number : 27886568
Accession Number : NP_071451
Short Description : (IN) Melanoma differentiation-associated protein 5
1. Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.
2. Hiscott J, Nguyen T-LA, Arguello M, et al. Manipulation of the nuclear factor-B pathway and the innate immune response by viruses. Oncogene 2006; 25:6844-67.
3. Kang D, Gopalrishnan RV, Lin L, et al. Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene. Oncogene 2004; 23:1789-800.
4. Andrejeva J, Childs KS, Young DF, et al. The V proteins of the paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc. Natl. Acad. Sci. USA 2004; 101:17264-9.