Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. ORAI1 often migrates at a higher than expected molecular weight in SDS-PAGE. This antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.
Additional Names : ORAI1 (NT), Transmembrane protein 142A, TMEM142A, Calcium release-activated calcium channel protein 1
Left: Western blot analysis of ORAI1 in human ovary tissue lysate with ORAI1 antibody at 1 µg/ml in the (A) absence or (B) presence of blocking peptide.
Below:Immunohistochemistry of ORAI1 in human spleen tissue with ORAI1 antibody at 10 µg/ml.
Other Product Images
Source :ORAI1 antibody was raised against a 18 amino acid peptide from near the amino terminus of human ORAI1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ORAI1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : ORAI1 antibody can be used for detection of ORAI1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4041P - ORAI1 Peptide
Long-Term Storage : ORAI1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1316 - Human Ovary Tissue Lysate
2. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity : H, M
GI Number : 97180269
Accession Number : Q96D31
Short Description : (NT) a T cell calcium channel
1. Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
2. Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
3. Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins – a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.