The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Additional Names : TIM-1 (IN), T cell immunoglobulin and mucin domain containing protein 1, Hepatitis A virus cellular receptor 1, HAVcr-1, Kim-1
Left: Western blot analysis of TIM-1 in human uterus tissue lysate with TIM-1 antibody at (A) 1 and (B) 2 µg/ml.
Source :TIM-1 antibody was raised against a 16 amino acid peptide from near the center of human TIM-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TIM-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3811P - TIM-1 Peptide
Long-Term Storage : TIM-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1317 - Human Uterus Tissue Lysate
Species Reactivity : H, M, R
GI Number : 153085427
Accession Number : NP_036338
Short Description : (IN) T cell immunoglobulin and mucin domain containing protein 1
1. Feigelstock D, Thompson P, Mattoo P, et al. The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor. J. Virol. 1998; 72:6621-8.
2. Ichimura T, Bonventre JV, Bailly V, et al. Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. J. Biol. Chem. 1998; 273:4135-42.
3. Meyers JH, Sabatos CA, Chakravarti S, et al. The TIM family regulates autoimmune and allergic diseases. Trends Mol. Med. 2005; 11:362-9.
4. Meyers JH, Chakravarti S, Schlesinger D, et al. TIM-4 is the ligand for TIM-1, and the TIM-1-TIM4 interaction regulates T cell proliferation. Nat. Immunol. 2005; 6:455-64.