Thursday, March 3, 2011

KLRA1 Antibody

KLRA1 Antibody

Catalog# :4989

KLRA1 (also known and Ly49L) is a member of the LY49 family of receptors Natural killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells. At least three isoforms of KLRA1 are known to exist.

Additional Names : KLRA1 (CT), Killer cell lectin-like receptor subfamily A member 1, Ly49, Ly49L

Left:Western blot analysis of KLRA1 in 293 cell lysate with KLRA1 antibody at (A) 1 µg/ml and (B) 2 µg/ml.

Below:Immunohistochemistry of KLRA1 in human spleen tissue with KLRA1 antibody at 2.5 µg/ml.

Other Product Images

Source :KLRA1 antibody was raised against a 13 amino acid peptide near the carboxy terminus of human KLRA1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : KLRA1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4989P - KLRA1 Peptide
Long-Term Storage : KLRA1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1210 - 293 Cell Lysate
Species Reactivity : H, M, R
GI Number : 119616600
Accession Number : EAW96194
Short Description : (CT) Killer cell lectin-like receptor subfamily A member 1
1. Makrigiannis AP, Etzler J, Winkler-Pickett R, et al. Identification of the Ly49L protein: evidence for activating counterparts to inhibitory Ly49 proteins. J. Leuk. Biol. 2000; 68:765-71.
2. Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol. 1994; 153:1068-79.
3. Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med. 1994; 180:2287-95.

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