KLRA2 (also known as Ly49B) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA2 is thought to be an inhibitory receptor and associates with the protein tyrosine phosphatases SHP1, SHP2, and SHIP in transfected cells. This antibody is predicted to not cross-react with other LY49 receptors.
Additional Names : KLRA2, Killer cell lectin-like receptor subfamily A member 2, Ly49B
Left: Western blot analysis of KLRA2 in mouse spleen tissue lysate with KLRA2 antibody at (A) 0.5 and (B) 1 µg/ml.
Source :KLRA2 antibody was raised against a 14 amino acid peptide near the carboxy terminus of mouse KLRA2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : KLRA2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : KLRA2 antibody can be used for detection of KLRA2 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5017P - KLRA2 Peptide
Long-Term Storage : KLRA2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1406 - Mouse Spleen Tissue Lysate
Species Reactivity : M, R
GI Number : 2497636
Accession Number : Q60660
Short Description : Killer cell lectin-like receptor subfamily A member 2
1. Gosselin P, Lusignan Y, Brennan J, et al. The NK2.1 receptor is encoded by Ly-49C and its expression is regulated by MHC class I alleles. Int. Immunol. 1997; 533-40.
2. Smith HR, Karlhofer FM, and Yokoyama WM. Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol. 1994; 153:1068-79.
3. Brennan J, Mager D, Jefferies W, et al. Expression of different members of the Ly-49 gene family defines distinct natural killer cell subsets and cell adhesion properties. J. Exp. Med. 1994; 180:2287-95.
4. Saleh A, Davies GE, Pascal V, et al. Identification of probabilistic transcriptional switches in the Ly49 gene cluster: a eukaryotic mechanism for selective gene activation. Immunity 2004; 21:55-66.