Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble (1). The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells (2). Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions (1,3). It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues (4,5).
Additional Names : Lano
Left: Western blot analysis of Lano in PC-3 whole cell lysate with Lano antibody at (A) 1 or (B) 2 µg /ml.
Below:Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/ml.
Other Product Images
Source :Lano antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Lano antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen : Human Lano Peptide (Cat. No. 3001P)
Application : Lano antibody can be used for detection of Lano by Western blot at 1 -2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat. No. 3001P - Lano Peptide
Long-Term Storage : Lano antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1216 - PC-3 Cell Lysate
2. Cat. No. 1320 - Human Colon Tissue Lysate
Species Reactivity : H
GI Number : 14701834
Accession Number : AAK72246
Short Description : a member of the LAP family
1. Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5.
2. Legouis R, Jaulin-Bastard F, Schott S, et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 2003; 4:1096-102.
3. Funke L, Dakoji S, Bredt DS. Membrane-Associated Guanylate Kinases Regulate Adhesion and Plasticity at Cell Junctions. Annu. Rev. Biochem. 2005; 74:219-45.
4. Bilder D and Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000; 403:676-80.