Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins such as RP105, a recently identified homolog of TLR4. This protein, in association with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway.. While MD-2 can directly bind to LPS, one of the activating molecules of TLR4, the function of MD-1 is less well-known. It has been suggested however, that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes.
Additional Names : MD-1 (CT), Lymphocyte antigen 86
Left: Western blot analysis of MD-1 in Daudi cell lysate with MD-1 antibody at (A) 0.5, (B) 1 and (C) 2 µg/ml.
Below:Immunohistochemistry of MD-1 in human spleen tissue with MD-1 antibody at 2 µg/ml.
Other Product Images
Source :MD-1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human MD-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : MD-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : MD-1 antibody can be used for the detection of MD-1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3847P - MD-1 Peptide
Long-Term Storage : MD-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity : H
GI Number : 4758708
Accession Number : NP_004262
Short Description : (CT) Toll-like receptor, TLR2, TLR4, RP105, MD-2
1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
2. Divanovic S, Trompette A, Atabani SF, et al. Inhibition of TLR-4/MD-2 signaling by RP105/MD-1. J. Endotoxin Res. 2005; 11:363-8.
3. Tsuneyoshi N, Fukudome K, Kohara J, et al. The functional and structural properties of MD-2 required for liposaccharide binding are absent in MD-1. J. Immunol. 2005; 174:340-4.
4. Nagai Y, Kobayashi T, Motoi Y, et al. The radioprotective 105/MD-1 complex links TLR2 and TLR4/MD-2 in antibody response to microbial membranes. J. Immunol. 2005; 174:7043-9.